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Crystallization and preliminary X-ray investigation of Escherichia coli porphobilinogen deaminase

Research output: Contribution to journalArticle

  • Peter M. Jordan
  • Martin J. Warren
  • Bob I. Mgbeje
  • Professor Steve Wood
  • Jonathan B. Cooper
  • Gordon Louie
  • Paul Brownlie
  • Richard Lambert
  • Thomas L. Blundell
Porphobilinogen deaminase, the polymerase that catalyses the synthesis of preuroporphyrinogen, the linear tetrapyrrole precursor of uroporphyrinogen III, has been crystallized from sodium acetate buffer with polyethylene glycol 6000 as precipitant. The crystals are orthorhombic and the space group is P21212, with unit cell dimensions a = 88.01 Å, b = 75.86 Å, c = 50.53 Å and α = β = gg = 90 °, indicating a single molecule of 34 kDa in the asymmetric unit. The crystals grow to dimensions of 1 mm × 2 mm × 0.5 mm within two weeks in the dark and are stable in the X-ray beam for at least 40 hours. Diffraction data beyond 1.7 Å resolution, observed with a synchrotron radiation source, indicate that a high resolution structure analysis is feasible.
Original languageEnglish
Pages (from-to)269-271
JournalJournal of Molecular Biology
Volume224
Issue number1
DOIs
Publication statusPublished - 1 Mar 1992

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