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Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro

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BACKGROUND: Effective treatments for glioblastoma multiforme (GBM) are lacking due, in part, to cellular heterogeneity. Consequently, single-target therapeutic strategies are unlikely to succeed. Simultaneous targeting of different neoplastic cell populations within the same tumour may, therefore, prove of value. Neuron-glia 2 (NG2), a transmembrane chondroitin sulphate proteoglycan, present on developing glial cells, and GD3(A), a ganglioside expressed on developing migratory glia, are re-expressed in GBM.

MATERIALS AND METHODS: The aims of this study were to conduct 'proof of concept' experiments in human GBM cell lines to show that proliferative high NG2-expressing cells and high GD3(A) -expressing migratory cells could be effectively ablated using a Mab-Zap saporin immunotoxin system.

RESULTS: The combinatorial ablation of both NG2 and GD3(A)-expressing cells resulted in significant reduction in GBM cell viability compared to single epitope targeting and controls (p<0.0001); non-neoplastic astrocytes were not affected.

CONCLUSION: Multiple targeting of GBM sub-populations may, therefore, help inform novel therapeutic approaches.

Original languageEnglish
Pages (from-to)77-84
JournalAnticancer Research
Volume35
Issue number1
Publication statusPublished - Jan 2015

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  • Dual Targeting NG2

    Rights statement: Open Access

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    Licence: CC BY

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