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Hierarchical electrospun tendon-ligament bioinspired scaffolds induce changes in fibroblasts morphology under static and dynamic conditions

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The regeneration of injured tendons and ligaments is challenging since the scaffolds needs proper mechanical properties and a biomimetic morphology. In particular, the morphological arrangement of scaffolds is a key point to drive the cells growth to properly regenerate the collagen extracellular matrix. Electrospinning is a promising technique to produce hierarchically structured nanofibrous scaffolds able to guide cells in the regeneration of the injured tissue. Moreover, the dynamic stretching in bioreactors of electrospun scaffolds had demonstrated to speed up cell shape modifications in vitro. The aim of the present study was to combine different imaging techniques such as high-resolution X-ray tomography (XCT), scanning electron microscopy (SEM), fluorescence microscopy and histology to investigate if hierarchically structured poly(L-lactic acid) and collagen electrospun scaffolds can induce morphological modifications in human fibroblasts, while cultured in static and dynamic conditions. After 7 days of parallel cultures, the results assessed that fibroblasts had proliferated on the external nanofibrous sheath of the static scaffolds, elongating themselves circumferentially. The dynamic cultures revealed a preferential axial orientation of fibroblasts growth on the external sheath. The aligned nanofiber bundles inside the hierarchical scaffolds instead, allowed a physiological distribution of the fibroblasts along the nanofiber direction. Inside the dynamic scaffolds, cells appeared thinner compared with the static counterpart. This study had demonstrated that hierarchically structured electrospun scaffolds can induce different fibroblasts morphological modifications during static and dynamic conditions, modifying their shape in the direction of the applied loads.
Original languageEnglish
JournalJournal of Microscopy
Early online date24 Jul 2019
DOIs
Publication statusEarly online - 24 Jul 2019

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  • Hierarchical electrospun tendon-ligament bioinspired scaffolds induce changes in fibroblasts morphology under static and dynamic conditions AAM

    Rights statement: This is the peer reviewed version of the following article: SENSINI, A. , CRISTOFOLINI, L. , ZUCCHELLI, A. , FOCARETE, M. , GUALANDI, C. , DE MORI, A. , KAO, A. , ROLDO, M. , BLUNN, G. and TOZZI, G. (2019), Hierarchical electrospun tendon‐ligament bioinspired scaffolds induce changes in fibroblasts morphology under static and dynamic conditions. Journal of Microscopy, which has been published in final form at https://doi.org/10.1111/jmi.12827. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

    Accepted author manuscript (Post-print), 1.38 MB, PDF document

    Due to publisher’s copyright restrictions, this document is not freely available to download from this website until: 24/07/20

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