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High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro

Research output: Contribution to journalArticlepeer-review

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High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro. / Read, Chris; Larose, Anne Marie; Leblanc, Benoît; Bannister, Andrew J.; Firek, Simon; Smith, Duncan R.; Moss, Tom.

In: Journal of Biological Chemistry, Vol. 267, No. 16, 05.06.1992, p. 10961-10967.

Research output: Contribution to journalArticlepeer-review

Harvard

Read, C, Larose, AM, Leblanc, B, Bannister, AJ, Firek, S, Smith, DR & Moss, T 1992, 'High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro', Journal of Biological Chemistry, vol. 267, no. 16, pp. 10961-10967. <http://www.jbc.org/content/267/16/10961.abstract>

APA

Read, C., Larose, A. M., Leblanc, B., Bannister, A. J., Firek, S., Smith, D. R., & Moss, T. (1992). High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro. Journal of Biological Chemistry, 267(16), 10961-10967. http://www.jbc.org/content/267/16/10961.abstract

Vancouver

Read C, Larose AM, Leblanc B, Bannister AJ, Firek S, Smith DR et al. High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro. Journal of Biological Chemistry. 1992 Jun 5;267(16):10961-10967.

Author

Read, Chris ; Larose, Anne Marie ; Leblanc, Benoît ; Bannister, Andrew J. ; Firek, Simon ; Smith, Duncan R. ; Moss, Tom. / High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 16. pp. 10961-10967.

Bibtex

@article{0a76e2902e9947a1aa2741c77f2a44f2,
title = "High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro",
abstract = "The first high resolution maps of the Xenopus laevis ribosomal promoter and its flanking regions (-179 to +14) have been created by assaying point mutants both in oocyte and in vitro. Within the promoter boundaries (-141(-145) to +3(+4)), domains analogous to the Core Promoter and {"}Upstream Control Element{"} (UCE) were clearly detected. The base pairs at -133, within the UCE, and -20, -10, -7, and +3, within the Core, were all shown to be especially important for promotion. Between the Core and UCE, two central promoter elements (CPEs) were also resolved. Surprisingly, these CPEs did not correspond to the highly conserved enhancer homology (∼-70 to -110), but to CCCGGCC motifs immediately flanking it. Although xUBF was shown to be a limiting component for in vitro transcription, none of the point mutations was found to affect the interaction of this factor with the promoter.",
author = "Chris Read and Larose, {Anne Marie} and Beno{\^i}t Leblanc and Bannister, {Andrew J.} and Simon Firek and Smith, {Duncan R.} and Tom Moss",
year = "1992",
month = jun,
day = "5",
language = "English",
volume = "267",
pages = "10961--10967",
journal = "The Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "16",

}

RIS

TY - JOUR

T1 - High resolution studies of the Xenopus laevis ribosomal gene promoter in vivo and in vitro

AU - Read, Chris

AU - Larose, Anne Marie

AU - Leblanc, Benoît

AU - Bannister, Andrew J.

AU - Firek, Simon

AU - Smith, Duncan R.

AU - Moss, Tom

PY - 1992/6/5

Y1 - 1992/6/5

N2 - The first high resolution maps of the Xenopus laevis ribosomal promoter and its flanking regions (-179 to +14) have been created by assaying point mutants both in oocyte and in vitro. Within the promoter boundaries (-141(-145) to +3(+4)), domains analogous to the Core Promoter and "Upstream Control Element" (UCE) were clearly detected. The base pairs at -133, within the UCE, and -20, -10, -7, and +3, within the Core, were all shown to be especially important for promotion. Between the Core and UCE, two central promoter elements (CPEs) were also resolved. Surprisingly, these CPEs did not correspond to the highly conserved enhancer homology (∼-70 to -110), but to CCCGGCC motifs immediately flanking it. Although xUBF was shown to be a limiting component for in vitro transcription, none of the point mutations was found to affect the interaction of this factor with the promoter.

AB - The first high resolution maps of the Xenopus laevis ribosomal promoter and its flanking regions (-179 to +14) have been created by assaying point mutants both in oocyte and in vitro. Within the promoter boundaries (-141(-145) to +3(+4)), domains analogous to the Core Promoter and "Upstream Control Element" (UCE) were clearly detected. The base pairs at -133, within the UCE, and -20, -10, -7, and +3, within the Core, were all shown to be especially important for promotion. Between the Core and UCE, two central promoter elements (CPEs) were also resolved. Surprisingly, these CPEs did not correspond to the highly conserved enhancer homology (∼-70 to -110), but to CCCGGCC motifs immediately flanking it. Although xUBF was shown to be a limiting component for in vitro transcription, none of the point mutations was found to affect the interaction of this factor with the promoter.

UR - http://www.scopus.com/inward/record.url?scp=0026687611&partnerID=8YFLogxK

M3 - Article

C2 - 1597438

AN - SCOPUS:0026687611

VL - 267

SP - 10961

EP - 10967

JO - The Journal of Biological Chemistry

JF - The Journal of Biological Chemistry

SN - 0021-9258

IS - 16

ER -

ID: 11466641