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High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions

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High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions. / Phillips, Jack; Butt, Louise; Henderson, Charlotte; Devonshire, Martin Adrian; Healy, Jess; Conway, Stuart J.; Locker, Nicolas; Pickford, Andrew; Vincent, Helen; Callaghan, Anastasia.

In: Nucleic Acids Research, Vol. 46, No. 14, e86, 21.08.2018.

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@article{d4a3142cac444ff387d72994c62f0a5e,
title = "High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions",
abstract = "We are just beginning to unravel the myriad of interactions in which non-coding RNAs participate. The intricate RNA interactome is the foundation of many biological processes, including bacterial virulence and human disease, and represents unexploited resources for the development of potential therapeutic interventions. However, identifying specific associations of a given RNA from the multitude of possible binding partners within the cell requires robust high-throughput systems for their rapid screening. Here, we present the first demonstration of functional-RNA arrays as a novel platform technology designed for the study of such interactions using immobilised, active RNAs. We have generated high-density RNA arrays by an innovative method involving surface-capture of in vitro transcribed RNAs. This approach has significant advantages over existing technologies, particularly in its versatility in regards to binding partner character. Indeed, proof-of-principle application of RNA arrays to both RNA-small molecule and RNA-RNA pairings is demonstrated, highlighting their potential as a platform technology for mapping RNA-based networks and for pharmaceutical screening. Furthermore, the simplicity of the method supports greateruser-accessibility over currently available technologies. We anticipate that functional-RNA arrays will find broad utility in the expanding field of RNA characterisation.",
keywords = "RCUK, BBSRC, BB/I532988/1",
author = "Jack Phillips and Louise Butt and Charlotte Henderson and Devonshire, {Martin Adrian} and Jess Healy and Conway, {Stuart J.} and Nicolas Locker and Andrew Pickford and Helen Vincent and Anastasia Callaghan",
year = "2018",
month = aug,
day = "21",
doi = "10.1093/nar/gky410",
language = "English",
volume = "46",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "14",

}

RIS

TY - JOUR

T1 - High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions

AU - Phillips, Jack

AU - Butt, Louise

AU - Henderson, Charlotte

AU - Devonshire, Martin Adrian

AU - Healy, Jess

AU - Conway, Stuart J.

AU - Locker, Nicolas

AU - Pickford, Andrew

AU - Vincent, Helen

AU - Callaghan, Anastasia

PY - 2018/8/21

Y1 - 2018/8/21

N2 - We are just beginning to unravel the myriad of interactions in which non-coding RNAs participate. The intricate RNA interactome is the foundation of many biological processes, including bacterial virulence and human disease, and represents unexploited resources for the development of potential therapeutic interventions. However, identifying specific associations of a given RNA from the multitude of possible binding partners within the cell requires robust high-throughput systems for their rapid screening. Here, we present the first demonstration of functional-RNA arrays as a novel platform technology designed for the study of such interactions using immobilised, active RNAs. We have generated high-density RNA arrays by an innovative method involving surface-capture of in vitro transcribed RNAs. This approach has significant advantages over existing technologies, particularly in its versatility in regards to binding partner character. Indeed, proof-of-principle application of RNA arrays to both RNA-small molecule and RNA-RNA pairings is demonstrated, highlighting their potential as a platform technology for mapping RNA-based networks and for pharmaceutical screening. Furthermore, the simplicity of the method supports greateruser-accessibility over currently available technologies. We anticipate that functional-RNA arrays will find broad utility in the expanding field of RNA characterisation.

AB - We are just beginning to unravel the myriad of interactions in which non-coding RNAs participate. The intricate RNA interactome is the foundation of many biological processes, including bacterial virulence and human disease, and represents unexploited resources for the development of potential therapeutic interventions. However, identifying specific associations of a given RNA from the multitude of possible binding partners within the cell requires robust high-throughput systems for their rapid screening. Here, we present the first demonstration of functional-RNA arrays as a novel platform technology designed for the study of such interactions using immobilised, active RNAs. We have generated high-density RNA arrays by an innovative method involving surface-capture of in vitro transcribed RNAs. This approach has significant advantages over existing technologies, particularly in its versatility in regards to binding partner character. Indeed, proof-of-principle application of RNA arrays to both RNA-small molecule and RNA-RNA pairings is demonstrated, highlighting their potential as a platform technology for mapping RNA-based networks and for pharmaceutical screening. Furthermore, the simplicity of the method supports greateruser-accessibility over currently available technologies. We anticipate that functional-RNA arrays will find broad utility in the expanding field of RNA characterisation.

KW - RCUK

KW - BBSRC

KW - BB/I532988/1

U2 - 10.1093/nar/gky410

DO - 10.1093/nar/gky410

M3 - Article

VL - 46

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 14

M1 - e86

ER -

ID: 10357491