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Identifying conserved polychaete molecular markers of metal exposure: Comparative analyses using the Alitta virens (Annelida, Lophotrochozoa) transcriptome

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Identifying conserved polychaete molecular markers of metal exposure: Comparative analyses using the Alitta virens (Annelida, Lophotrochozoa) transcriptome. / Green Etxabe, Amaia; Pini, Jennifer M.; Short, Stephen; Cunha, Luis; Kille, Peter; Watson, Gordon J.

In: Comparative Biochemistry and Physiology - Part C Toxicology & Pharmacology, Vol. 240, 108913, 01.02.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

Green Etxabe, A, Pini, JM, Short, S, Cunha, L, Kille, P & Watson, GJ 2021, 'Identifying conserved polychaete molecular markers of metal exposure: Comparative analyses using the Alitta virens (Annelida, Lophotrochozoa) transcriptome', Comparative Biochemistry and Physiology - Part C Toxicology & Pharmacology, vol. 240, 108913. https://doi.org/10.1016/j.cbpc.2020.108913

APA

Green Etxabe, A., Pini, J. M., Short, S., Cunha, L., Kille, P., & Watson, G. J. (2021). Identifying conserved polychaete molecular markers of metal exposure: Comparative analyses using the Alitta virens (Annelida, Lophotrochozoa) transcriptome. Comparative Biochemistry and Physiology - Part C Toxicology & Pharmacology, 240, [108913]. https://doi.org/10.1016/j.cbpc.2020.108913

Vancouver

Green Etxabe A, Pini JM, Short S, Cunha L, Kille P, Watson GJ. Identifying conserved polychaete molecular markers of metal exposure: Comparative analyses using the Alitta virens (Annelida, Lophotrochozoa) transcriptome. Comparative Biochemistry and Physiology - Part C Toxicology & Pharmacology. 2021 Feb 1;240. 108913. https://doi.org/10.1016/j.cbpc.2020.108913

Author

Green Etxabe, Amaia ; Pini, Jennifer M. ; Short, Stephen ; Cunha, Luis ; Kille, Peter ; Watson, Gordon J. / Identifying conserved polychaete molecular markers of metal exposure: Comparative analyses using the Alitta virens (Annelida, Lophotrochozoa) transcriptome. In: Comparative Biochemistry and Physiology - Part C Toxicology & Pharmacology. 2021 ; Vol. 240.

Bibtex

@article{9b06101e7cf84157bed2da7a95f022c1,
title = "Identifying conserved polychaete molecular markers of metal exposure: Comparative analyses using the Alitta virens (Annelida, Lophotrochozoa) transcriptome",
abstract = "Polychaetes are vital for evaluating the effects of toxic metals in marine systems, and sensitive molecular biomarkers should be integral to monitoring efforts. However, the few polychaete markers that exist are inconsistent, even within the same species, failing to identify gene expression changes in metal-exposed animals incurring clear metabolic costs. Comparing previously characterised polychaete metal-responsive genes with those of another carefully selected species could identify biomarkers applicable across polychaetes. The ragworm Alitta virens (Sars, 1835) is particularly suited for such comparisons due to its dominance of fully saline coastal areas, widespread distribution, large biomass, and its phylogenetic position relative to other polychaete {\textquoteleft}omic{\textquoteright} resources. A transcriptome atlas for A. virens was generated and an RNASeq-qPCR screening approach was used to characterise the response to chronic exposures of environmentally relevant concentrations of copper and zinc in controlled mesocosms. Genes presenting dramatic expression changes in A. virens were compared with known metal-responsive genes in other polychaetes to identify new possible biomarkers and assess those currently used. This revealed some current markers should probably be abandoned (e.g. Atox1), while others, such as GST-Omega, should be used with caution, as different polychaete species appear to upregulate distinct GST-Omega orthologues. In addition, the comparisons give some indication of genes that are induced by metal exposure across phylogenetically divergent polychaetes, including a suite of haemoglobin subunits and linker chains that could play conserved roles in metal-stress response. Although such newly identified markers need further characterisation, they offer alternatives to current markers that are plainly insufficient.",
keywords = "ragworm, benthic, heavy metal, haemoglobin, polychaete, pollution",
author = "{Green Etxabe}, Amaia and Pini, {Jennifer M.} and Stephen Short and Luis Cunha and Peter Kille and Watson, {Gordon J.}",
year = "2021",
month = feb,
day = "1",
doi = "10.1016/j.cbpc.2020.108913",
language = "English",
volume = "240",
journal = "Comparative Biochemistry and Physiology - Part C Toxicology & Pharmacology",
issn = "1532-0456",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Identifying conserved polychaete molecular markers of metal exposure: Comparative analyses using the Alitta virens (Annelida, Lophotrochozoa) transcriptome

AU - Green Etxabe, Amaia

AU - Pini, Jennifer M.

AU - Short, Stephen

AU - Cunha, Luis

AU - Kille, Peter

AU - Watson, Gordon J.

PY - 2021/2/1

Y1 - 2021/2/1

N2 - Polychaetes are vital for evaluating the effects of toxic metals in marine systems, and sensitive molecular biomarkers should be integral to monitoring efforts. However, the few polychaete markers that exist are inconsistent, even within the same species, failing to identify gene expression changes in metal-exposed animals incurring clear metabolic costs. Comparing previously characterised polychaete metal-responsive genes with those of another carefully selected species could identify biomarkers applicable across polychaetes. The ragworm Alitta virens (Sars, 1835) is particularly suited for such comparisons due to its dominance of fully saline coastal areas, widespread distribution, large biomass, and its phylogenetic position relative to other polychaete ‘omic’ resources. A transcriptome atlas for A. virens was generated and an RNASeq-qPCR screening approach was used to characterise the response to chronic exposures of environmentally relevant concentrations of copper and zinc in controlled mesocosms. Genes presenting dramatic expression changes in A. virens were compared with known metal-responsive genes in other polychaetes to identify new possible biomarkers and assess those currently used. This revealed some current markers should probably be abandoned (e.g. Atox1), while others, such as GST-Omega, should be used with caution, as different polychaete species appear to upregulate distinct GST-Omega orthologues. In addition, the comparisons give some indication of genes that are induced by metal exposure across phylogenetically divergent polychaetes, including a suite of haemoglobin subunits and linker chains that could play conserved roles in metal-stress response. Although such newly identified markers need further characterisation, they offer alternatives to current markers that are plainly insufficient.

AB - Polychaetes are vital for evaluating the effects of toxic metals in marine systems, and sensitive molecular biomarkers should be integral to monitoring efforts. However, the few polychaete markers that exist are inconsistent, even within the same species, failing to identify gene expression changes in metal-exposed animals incurring clear metabolic costs. Comparing previously characterised polychaete metal-responsive genes with those of another carefully selected species could identify biomarkers applicable across polychaetes. The ragworm Alitta virens (Sars, 1835) is particularly suited for such comparisons due to its dominance of fully saline coastal areas, widespread distribution, large biomass, and its phylogenetic position relative to other polychaete ‘omic’ resources. A transcriptome atlas for A. virens was generated and an RNASeq-qPCR screening approach was used to characterise the response to chronic exposures of environmentally relevant concentrations of copper and zinc in controlled mesocosms. Genes presenting dramatic expression changes in A. virens were compared with known metal-responsive genes in other polychaetes to identify new possible biomarkers and assess those currently used. This revealed some current markers should probably be abandoned (e.g. Atox1), while others, such as GST-Omega, should be used with caution, as different polychaete species appear to upregulate distinct GST-Omega orthologues. In addition, the comparisons give some indication of genes that are induced by metal exposure across phylogenetically divergent polychaetes, including a suite of haemoglobin subunits and linker chains that could play conserved roles in metal-stress response. Although such newly identified markers need further characterisation, they offer alternatives to current markers that are plainly insufficient.

KW - ragworm

KW - benthic

KW - heavy metal

KW - haemoglobin

KW - polychaete

KW - pollution

UR - https://linkinghub.elsevier.com/retrieve/pii/S1532045620302131

U2 - 10.1016/j.cbpc.2020.108913

DO - 10.1016/j.cbpc.2020.108913

M3 - Article

VL - 240

JO - Comparative Biochemistry and Physiology - Part C Toxicology & Pharmacology

JF - Comparative Biochemistry and Physiology - Part C Toxicology & Pharmacology

SN - 1532-0456

M1 - 108913

ER -

ID: 23347989