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Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus

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Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus. / Feio, M.; Beech, I.; Carepo, M.; Lopes, J.; Cheung, C.; Franco, R.; Guezennec, J.; Smith, James; Mitchell, Julian; Moura, J.; Lino, A.

In: Anaerobe, Vol. 4, No. 2, 1998, p. 117-130.

Research output: Contribution to journalArticlepeer-review

Harvard

Feio, M, Beech, I, Carepo, M, Lopes, J, Cheung, C, Franco, R, Guezennec, J, Smith, J, Mitchell, J, Moura, J & Lino, A 1998, 'Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus', Anaerobe, vol. 4, no. 2, pp. 117-130.

APA

Feio, M., Beech, I., Carepo, M., Lopes, J., Cheung, C., Franco, R., Guezennec, J., Smith, J., Mitchell, J., Moura, J., & Lino, A. (1998). Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus. Anaerobe, 4(2), 117-130.

Vancouver

Feio M, Beech I, Carepo M, Lopes J, Cheung C, Franco R et al. Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus. Anaerobe. 1998;4(2):117-130.

Author

Feio, M. ; Beech, I. ; Carepo, M. ; Lopes, J. ; Cheung, C. ; Franco, R. ; Guezennec, J. ; Smith, James ; Mitchell, Julian ; Moura, J. ; Lino, A. / Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus. In: Anaerobe. 1998 ; Vol. 4, No. 2. pp. 117-130.

Bibtex

@article{20aa4a9b68624495bae409fadc408a97,
title = "Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus",
abstract = "A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gramnegative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c(3), and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria. On the basis of the results obtained, it is suggested that Ind 1 belongs to a new species of the genus Desulfovibrio, and the name Desulfovibrio indonensis is proposed.",
author = "M. Feio and I. Beech and M. Carepo and J. Lopes and C. Cheung and R. Franco and J. Guezennec and James Smith and Julian Mitchell and J. Moura and A. Lino",
year = "1998",
language = "English",
volume = "4",
pages = "117--130",
journal = "Anaerobe",
issn = "1075-9964",
publisher = "Academic Press Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus

AU - Feio, M.

AU - Beech, I.

AU - Carepo, M.

AU - Lopes, J.

AU - Cheung, C.

AU - Franco, R.

AU - Guezennec, J.

AU - Smith, James

AU - Mitchell, Julian

AU - Moura, J.

AU - Lino, A.

PY - 1998

Y1 - 1998

N2 - A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gramnegative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c(3), and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria. On the basis of the results obtained, it is suggested that Ind 1 belongs to a new species of the genus Desulfovibrio, and the name Desulfovibrio indonensis is proposed.

AB - A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gramnegative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c(3), and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria. On the basis of the results obtained, it is suggested that Ind 1 belongs to a new species of the genus Desulfovibrio, and the name Desulfovibrio indonensis is proposed.

M3 - Article

VL - 4

SP - 117

EP - 130

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

IS - 2

ER -

ID: 75743