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Knockout and knock-in mouse models to study purinergic signaling

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Purinergic signaling involves extracellular purines and pyrimidines acting upon specific cell surface purinoceptors classified into the P1, P2X, and P2Y families for nucleosides and nucleotides. This widespread signaling mechanism is active in all major tissues and influences a range of functions in health and disease. Orthologs to all but one of the human purinoceptors have been found in mouse, making this laboratory animal a useful model to study their function. Indeed, analyses of purinoceptors via knock-in or knockout approaches to produce gain or loss of function phenotypes have revealed several important therapeutic targets. None of the homozygous purinoceptor knockouts proved to be developmentally lethal, which suggest that either these receptors are not involved in key developmental processes or that the large number of receptors in each family allowed for functional compensation. Different models for the same purinoceptor often show compatible phenotypes but there have been examples of significant discrepancies. These revealed unexpected differences in the structure of human and mouse genes and emphasized the importance of the genetic background of different mouse strains. In this chapter, we provide an overview of the current knowledge and new trends in the modifications of purinoceptor genes in vivo. We discuss the resulting phenotypes, their applications and relative merits and limitations of mouse models available to study purinoceptor subtypes.
Original languageEnglish
Title of host publicationPurinergic Signalling: Methods and Protocols
EditorsPablo Pelegrín
PublisherSpringer
Chapter2
Pages17-43
Number of pages27
ISBN (Electronic)978-1-4939-9717-6
ISBN (Print)978-1-4939-9716-9
DOIs
Publication statusEarly online - 24 Oct 2019

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume2041
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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