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Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons

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Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons. / de Almeida, Monique Marylin Alves; Souza, Cleide dos Santos; Dourado, Naiara Silva; da Silva, Alessandra Bispo; Ferreira, Rafael Short; David, Jorge Mauricio; David, Juceni Pereira; Costa, Maria de Fátima Dias; da Silva, Victor Diógenes Amaral; Butt, Arthur Morgan; Costa, Silvia Lima.

In: Biomolecules, Vol. 10, No. 4, 562, 07.04.2020.

Research output: Contribution to journalArticle

Harvard

de Almeida, MMA, Souza, CDS, Dourado, NS, da Silva, AB, Ferreira, RS, David, JM, David, JP, Costa, MDFD, da Silva, VDA, Butt, AM & Costa, SL 2020, 'Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons', Biomolecules, vol. 10, no. 4, 562. https://doi.org/10.3390/biom10040562

APA

de Almeida, M. M. A., Souza, C. D. S., Dourado, N. S., da Silva, A. B., Ferreira, R. S., David, J. M., David, J. P., Costa, M. D. F. D., da Silva, V. D. A., Butt, A. M., & Costa, S. L. (2020). Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons. Biomolecules, 10(4), [562]. https://doi.org/10.3390/biom10040562

Vancouver

de Almeida MMA, Souza CDS, Dourado NS, da Silva AB, Ferreira RS, David JM et al. Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons. Biomolecules. 2020 Apr 7;10(4). 562. https://doi.org/10.3390/biom10040562

Author

de Almeida, Monique Marylin Alves ; Souza, Cleide dos Santos ; Dourado, Naiara Silva ; da Silva, Alessandra Bispo ; Ferreira, Rafael Short ; David, Jorge Mauricio ; David, Juceni Pereira ; Costa, Maria de Fátima Dias ; da Silva, Victor Diógenes Amaral ; Butt, Arthur Morgan ; Costa, Silvia Lima. / Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons. In: Biomolecules. 2020 ; Vol. 10, No. 4.

Bibtex

@article{87de353df4764b78abefc8516523a5f9,
title = "Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons",
abstract = "Inflammation and oxidative stress are common aspects of most neurodegenerative diseases in the central nervous system. In this context, microglia and astrocytes are central to mediating the balance between neuroprotective and neurodestructive mechanisms. Flavonoids have potent anti-inflammatory and antioxidant properties. Here, we have examined the anti-inflammatory and neuroprotective potential of the flavonoid agathisflavone (FAB), which is derived from the Brazilian plant Poincianella pyramidalis, in in vitro models of neuroinflammation. Cocultures of neurons/glial cells were exposed to lipopolysaccharide (LPS, 1 µg/mL) or interleukin (IL)-1β (10 ng/mL) for 24 h and treated with FAB (0.1 and 1 µM, 24 h). FAB displayed a significant neuroprotective effect, as measured by nitric oxide (NO) production, Fluoro-Jade B (FJ-B) staining, and immunocytochemistry (ICC) for the neuronal marker β-tubulin and the cell death marker caspase-3, preserving neuronal soma and increasing neurite outgrowth. FAB significantly decreased the LPS-induced microglial proliferation, identified by ICC for Iba-1/bromodeoxyuridine (BrdU) and CD68 (microglia M1 profile marker). In contrast, FAB had no apparent effect on astrocytes, as determined by ICC for glial fibrillary acidic protein (GFAP). Furthermore, FAB protected against the cytodestructive and proinflammatory effects of IL-1β, a key cytokine that is released by activated microglia and astrocytes, and ICC showed that combined treatment of FAB with α and β estrogen receptor antagonists did not affect NF-κB expression. In addition, qPCR analysis demonstrated that FAB decreased the expression of proinflammatory molecules TNF-α, IL-1β, and connexins CCL5 and CCL2, as well as increased the expression of the regulatory molecule IL-10. Together, these findings indicate that FAB has a significant neuroprotective and anti-inflammatory effect in vitro, which may be considered as an adjuvant for the treatment of neurodegenerative diseases. ",
keywords = "flavonoids, agathisflavone, neuroprotection, anti-neuroinflammation, RCUK, BBSRC, BB/M029379/1",
author = "{de Almeida}, {Monique Marylin Alves} and Souza, {Cleide dos Santos} and Dourado, {Naiara Silva} and {da Silva}, {Alessandra Bispo} and Ferreira, {Rafael Short} and David, {Jorge Mauricio} and David, {Juceni Pereira} and Costa, {Maria de F{\'a}tima Dias} and {da Silva}, {Victor Di{\'o}genes Amaral} and Butt, {Arthur Morgan} and Costa, {Silvia Lima}",
year = "2020",
month = apr,
day = "7",
doi = "10.3390/biom10040562",
language = "English",
volume = "10",
journal = "Biomolecules",
issn = "2218-273X",
publisher = "Multidisciplinary Digital Publishing Institute",
number = "4",

}

RIS

TY - JOUR

T1 - Phytoestrogen agathisflavone ameliorates neuroinflammation-induced by LPS and IL-1β and protects neurons in cocultures of glia/neurons

AU - de Almeida, Monique Marylin Alves

AU - Souza, Cleide dos Santos

AU - Dourado, Naiara Silva

AU - da Silva, Alessandra Bispo

AU - Ferreira, Rafael Short

AU - David, Jorge Mauricio

AU - David, Juceni Pereira

AU - Costa, Maria de Fátima Dias

AU - da Silva, Victor Diógenes Amaral

AU - Butt, Arthur Morgan

AU - Costa, Silvia Lima

PY - 2020/4/7

Y1 - 2020/4/7

N2 - Inflammation and oxidative stress are common aspects of most neurodegenerative diseases in the central nervous system. In this context, microglia and astrocytes are central to mediating the balance between neuroprotective and neurodestructive mechanisms. Flavonoids have potent anti-inflammatory and antioxidant properties. Here, we have examined the anti-inflammatory and neuroprotective potential of the flavonoid agathisflavone (FAB), which is derived from the Brazilian plant Poincianella pyramidalis, in in vitro models of neuroinflammation. Cocultures of neurons/glial cells were exposed to lipopolysaccharide (LPS, 1 µg/mL) or interleukin (IL)-1β (10 ng/mL) for 24 h and treated with FAB (0.1 and 1 µM, 24 h). FAB displayed a significant neuroprotective effect, as measured by nitric oxide (NO) production, Fluoro-Jade B (FJ-B) staining, and immunocytochemistry (ICC) for the neuronal marker β-tubulin and the cell death marker caspase-3, preserving neuronal soma and increasing neurite outgrowth. FAB significantly decreased the LPS-induced microglial proliferation, identified by ICC for Iba-1/bromodeoxyuridine (BrdU) and CD68 (microglia M1 profile marker). In contrast, FAB had no apparent effect on astrocytes, as determined by ICC for glial fibrillary acidic protein (GFAP). Furthermore, FAB protected against the cytodestructive and proinflammatory effects of IL-1β, a key cytokine that is released by activated microglia and astrocytes, and ICC showed that combined treatment of FAB with α and β estrogen receptor antagonists did not affect NF-κB expression. In addition, qPCR analysis demonstrated that FAB decreased the expression of proinflammatory molecules TNF-α, IL-1β, and connexins CCL5 and CCL2, as well as increased the expression of the regulatory molecule IL-10. Together, these findings indicate that FAB has a significant neuroprotective and anti-inflammatory effect in vitro, which may be considered as an adjuvant for the treatment of neurodegenerative diseases.

AB - Inflammation and oxidative stress are common aspects of most neurodegenerative diseases in the central nervous system. In this context, microglia and astrocytes are central to mediating the balance between neuroprotective and neurodestructive mechanisms. Flavonoids have potent anti-inflammatory and antioxidant properties. Here, we have examined the anti-inflammatory and neuroprotective potential of the flavonoid agathisflavone (FAB), which is derived from the Brazilian plant Poincianella pyramidalis, in in vitro models of neuroinflammation. Cocultures of neurons/glial cells were exposed to lipopolysaccharide (LPS, 1 µg/mL) or interleukin (IL)-1β (10 ng/mL) for 24 h and treated with FAB (0.1 and 1 µM, 24 h). FAB displayed a significant neuroprotective effect, as measured by nitric oxide (NO) production, Fluoro-Jade B (FJ-B) staining, and immunocytochemistry (ICC) for the neuronal marker β-tubulin and the cell death marker caspase-3, preserving neuronal soma and increasing neurite outgrowth. FAB significantly decreased the LPS-induced microglial proliferation, identified by ICC for Iba-1/bromodeoxyuridine (BrdU) and CD68 (microglia M1 profile marker). In contrast, FAB had no apparent effect on astrocytes, as determined by ICC for glial fibrillary acidic protein (GFAP). Furthermore, FAB protected against the cytodestructive and proinflammatory effects of IL-1β, a key cytokine that is released by activated microglia and astrocytes, and ICC showed that combined treatment of FAB with α and β estrogen receptor antagonists did not affect NF-κB expression. In addition, qPCR analysis demonstrated that FAB decreased the expression of proinflammatory molecules TNF-α, IL-1β, and connexins CCL5 and CCL2, as well as increased the expression of the regulatory molecule IL-10. Together, these findings indicate that FAB has a significant neuroprotective and anti-inflammatory effect in vitro, which may be considered as an adjuvant for the treatment of neurodegenerative diseases.

KW - flavonoids

KW - agathisflavone

KW - neuroprotection

KW - anti-neuroinflammation

KW - RCUK

KW - BBSRC

KW - BB/M029379/1

U2 - 10.3390/biom10040562

DO - 10.3390/biom10040562

M3 - Article

VL - 10

JO - Biomolecules

JF - Biomolecules

SN - 2218-273X

IS - 4

M1 - 562

ER -

ID: 20696883