Skip to content
Back to outputs

Reference intervals for absolute and percentage immature platelet fraction using the Sysmex XN-10 automated haematology analyser in a UK population

Research output: Contribution to journalArticlepeer-review

Standard

Reference intervals for absolute and percentage immature platelet fraction using the Sysmex XN-10 automated haematology analyser in a UK population. / Ali, Usman; Knight, Gavin; Gibbs, Roz; Tsitsikas, Dimitris A.

In: Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 77, No. 8, 01.12.2017, p. 658-664.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Ali, Usman ; Knight, Gavin ; Gibbs, Roz ; Tsitsikas, Dimitris A. / Reference intervals for absolute and percentage immature platelet fraction using the Sysmex XN-10 automated haematology analyser in a UK population. In: Scandinavian Journal of Clinical and Laboratory Investigation. 2017 ; Vol. 77, No. 8. pp. 658-664.

Bibtex

@article{ad1670dec81b477c99c3fe5869950b40,
title = "Reference intervals for absolute and percentage immature platelet fraction using the Sysmex XN-10 automated haematology analyser in a UK population",
abstract = "Background: Immature platelet fraction (IPF) estimation is a non-invasive and sensitive test that is available on recently introduced Sysmex XN-series of automated haematology analysers. It is a direct cellular indicator of thrombopoiesis. The aim of this study was to establish reference intervals for IPF, for both absolute (A-IPF) and percentage (%-IPF) measurements.Material and methods: A total of 2366 samples that met the inclusion criteria were assayed for full blood count on the Sysmex XN-10 and a non-parametric percentile method was used for calculating the reference intervals.Results: After the outliers were excluded, the reference interval for %-IPF and A-IPF on Sysmex XN-10 were 1.6–10.1% and 4.37–23.21 × 109/L in total individuals, respectively. There was a statistical significance noted between the sexes (p = .004) for %-IPF, therefore a sex-specific reference interval was established, which was 1.8–10.0% for the males and 1.5–10.1% for females. No significant difference in sex status for A-IPF and age status for both %-IPF and A-IPF was observed. A very poor correlation was estimated between age versus %-IPF, ρ = 0.0156, and age versus A-IPF, ρ = −0.0023, indicating that there is no overall biological relationship between age and these parameters. As expected, a strong correlation between %-IPF and A-IPF was noted which could be attributed to their inter-relatedness.Conclusions: This large-scale study showed comparable reference intervals with the previous studies for %-IPF and A-IPF in a UK population. It found the need to establish sex-specific reference intervals for %-IPF, but not for A-IPF, whereas reference intervals were found to be stable across the age range.",
keywords = "reference values, blood platelet, haematology, thrombocytopenia, blood cell count, haematologic tests, haematologic diseases, clinicla laboratory techniques, clinical laboratory services, UK",
author = "Usman Ali and Gavin Knight and Roz Gibbs and Tsitsikas, {Dimitris A.}",
year = "2017",
month = dec,
day = "1",
doi = "10.1080/00365513.2017.1394488",
language = "English",
volume = "77",
pages = "658--664",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
issn = "0036-5513",
publisher = "Taylor & Francis",
number = "8",

}

RIS

TY - JOUR

T1 - Reference intervals for absolute and percentage immature platelet fraction using the Sysmex XN-10 automated haematology analyser in a UK population

AU - Ali, Usman

AU - Knight, Gavin

AU - Gibbs, Roz

AU - Tsitsikas, Dimitris A.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Background: Immature platelet fraction (IPF) estimation is a non-invasive and sensitive test that is available on recently introduced Sysmex XN-series of automated haematology analysers. It is a direct cellular indicator of thrombopoiesis. The aim of this study was to establish reference intervals for IPF, for both absolute (A-IPF) and percentage (%-IPF) measurements.Material and methods: A total of 2366 samples that met the inclusion criteria were assayed for full blood count on the Sysmex XN-10 and a non-parametric percentile method was used for calculating the reference intervals.Results: After the outliers were excluded, the reference interval for %-IPF and A-IPF on Sysmex XN-10 were 1.6–10.1% and 4.37–23.21 × 109/L in total individuals, respectively. There was a statistical significance noted between the sexes (p = .004) for %-IPF, therefore a sex-specific reference interval was established, which was 1.8–10.0% for the males and 1.5–10.1% for females. No significant difference in sex status for A-IPF and age status for both %-IPF and A-IPF was observed. A very poor correlation was estimated between age versus %-IPF, ρ = 0.0156, and age versus A-IPF, ρ = −0.0023, indicating that there is no overall biological relationship between age and these parameters. As expected, a strong correlation between %-IPF and A-IPF was noted which could be attributed to their inter-relatedness.Conclusions: This large-scale study showed comparable reference intervals with the previous studies for %-IPF and A-IPF in a UK population. It found the need to establish sex-specific reference intervals for %-IPF, but not for A-IPF, whereas reference intervals were found to be stable across the age range.

AB - Background: Immature platelet fraction (IPF) estimation is a non-invasive and sensitive test that is available on recently introduced Sysmex XN-series of automated haematology analysers. It is a direct cellular indicator of thrombopoiesis. The aim of this study was to establish reference intervals for IPF, for both absolute (A-IPF) and percentage (%-IPF) measurements.Material and methods: A total of 2366 samples that met the inclusion criteria were assayed for full blood count on the Sysmex XN-10 and a non-parametric percentile method was used for calculating the reference intervals.Results: After the outliers were excluded, the reference interval for %-IPF and A-IPF on Sysmex XN-10 were 1.6–10.1% and 4.37–23.21 × 109/L in total individuals, respectively. There was a statistical significance noted between the sexes (p = .004) for %-IPF, therefore a sex-specific reference interval was established, which was 1.8–10.0% for the males and 1.5–10.1% for females. No significant difference in sex status for A-IPF and age status for both %-IPF and A-IPF was observed. A very poor correlation was estimated between age versus %-IPF, ρ = 0.0156, and age versus A-IPF, ρ = −0.0023, indicating that there is no overall biological relationship between age and these parameters. As expected, a strong correlation between %-IPF and A-IPF was noted which could be attributed to their inter-relatedness.Conclusions: This large-scale study showed comparable reference intervals with the previous studies for %-IPF and A-IPF in a UK population. It found the need to establish sex-specific reference intervals for %-IPF, but not for A-IPF, whereas reference intervals were found to be stable across the age range.

KW - reference values

KW - blood platelet

KW - haematology

KW - thrombocytopenia

KW - blood cell count

KW - haematologic tests

KW - haematologic diseases

KW - clinicla laboratory techniques

KW - clinical laboratory services

KW - UK

U2 - 10.1080/00365513.2017.1394488

DO - 10.1080/00365513.2017.1394488

M3 - Article

VL - 77

SP - 658

EP - 664

JO - Scandinavian Journal of Clinical and Laboratory Investigation

JF - Scandinavian Journal of Clinical and Laboratory Investigation

SN - 0036-5513

IS - 8

ER -

ID: 8077356