Skip to content
Back to outputs

Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes

Research output: Contribution to journalArticle

Standard

Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes. / Schneider, Robert; Bannister, Andrew J.; Myers, Fiona A.; Thorne, Alan W.; Crane-Robinson, Colyn; Kouzarides, Tony.

In: The Journal of Biological Chemistry, Vol. 280, No. 18, 06.05.2005, p. 17732-17736.

Research output: Contribution to journalArticle

Harvard

Schneider, R, Bannister, AJ, Myers, FA, Thorne, AW, Crane-Robinson, C & Kouzarides, T 2005, 'Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes', The Journal of Biological Chemistry, vol. 280, no. 18, pp. 17732-17736. https://doi.org/10.1074/jbc.M500796200

APA

Schneider, R., Bannister, A. J., Myers, F. A., Thorne, A. W., Crane-Robinson, C., & Kouzarides, T. (2005). Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes. The Journal of Biological Chemistry, 280(18), 17732-17736. https://doi.org/10.1074/jbc.M500796200

Vancouver

Schneider R, Bannister AJ, Myers FA, Thorne AW, Crane-Robinson C, Kouzarides T. Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes. The Journal of Biological Chemistry. 2005 May 6;280(18):17732-17736. https://doi.org/10.1074/jbc.M500796200

Author

Schneider, Robert ; Bannister, Andrew J. ; Myers, Fiona A. ; Thorne, Alan W. ; Crane-Robinson, Colyn ; Kouzarides, Tony. / Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes. In: The Journal of Biological Chemistry. 2005 ; Vol. 280, No. 18. pp. 17732-17736.

Bibtex

@article{9921e1f4b58a4d13bfff6fa6dcb4b12e,
title = "Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes",
abstract = "Methylation of lysine 4 of histone H3 (K4/H3) is linked to transcriptional activity, whereas methylation of K9/H3 is tightly associated with gene inactivity. These are well characterized sites of methylation within histones, but there are numerous other, less characterized, sites of modification. In Saccharomyces cerevisiae, methylation of K36/H3 has been linked to active genes, but little is known about this methylation in higher eukaryotes. Here we analyzed for the first time the levels and spatial distribution of di- and tri-methyl (di- and tri-Me) K36/H3 in metazoan genes. We analyzed chicken genes that are developmentally regulated, constitutively active, or inactive. We found that active genes contain high levels of these modifications compared with inactive genes. Furthermore, in actively transcribed regions the levels of di- and tri-Me K36/H3 peak toward the 3′ end of the gene. This is in striking contrast to the distributions of di- and tri-Me K4/H3, which peak early in actively transcribed regions. Thus, di/tri-Me K4/H3 and di/tri-Me K36/H3 are both useful markers of active genes, but their genic distribution indicates differing roles. Our data suggest that the unique spatial distribution of di- and tri-Me K36/H3 plays a role in transcriptional termination and/or early RNA processing.",
author = "Robert Schneider and Bannister, {Andrew J.} and Myers, {Fiona A.} and Thorne, {Alan W.} and Colyn Crane-Robinson and Tony Kouzarides",
year = "2005",
month = "5",
day = "6",
doi = "10.1074/jbc.M500796200",
language = "English",
volume = "280",
pages = "17732--17736",
journal = "The Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "18",

}

RIS

TY - JOUR

T1 - Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes

AU - Schneider, Robert

AU - Bannister, Andrew J.

AU - Myers, Fiona A.

AU - Thorne, Alan W.

AU - Crane-Robinson, Colyn

AU - Kouzarides, Tony

PY - 2005/5/6

Y1 - 2005/5/6

N2 - Methylation of lysine 4 of histone H3 (K4/H3) is linked to transcriptional activity, whereas methylation of K9/H3 is tightly associated with gene inactivity. These are well characterized sites of methylation within histones, but there are numerous other, less characterized, sites of modification. In Saccharomyces cerevisiae, methylation of K36/H3 has been linked to active genes, but little is known about this methylation in higher eukaryotes. Here we analyzed for the first time the levels and spatial distribution of di- and tri-methyl (di- and tri-Me) K36/H3 in metazoan genes. We analyzed chicken genes that are developmentally regulated, constitutively active, or inactive. We found that active genes contain high levels of these modifications compared with inactive genes. Furthermore, in actively transcribed regions the levels of di- and tri-Me K36/H3 peak toward the 3′ end of the gene. This is in striking contrast to the distributions of di- and tri-Me K4/H3, which peak early in actively transcribed regions. Thus, di/tri-Me K4/H3 and di/tri-Me K36/H3 are both useful markers of active genes, but their genic distribution indicates differing roles. Our data suggest that the unique spatial distribution of di- and tri-Me K36/H3 plays a role in transcriptional termination and/or early RNA processing.

AB - Methylation of lysine 4 of histone H3 (K4/H3) is linked to transcriptional activity, whereas methylation of K9/H3 is tightly associated with gene inactivity. These are well characterized sites of methylation within histones, but there are numerous other, less characterized, sites of modification. In Saccharomyces cerevisiae, methylation of K36/H3 has been linked to active genes, but little is known about this methylation in higher eukaryotes. Here we analyzed for the first time the levels and spatial distribution of di- and tri-methyl (di- and tri-Me) K36/H3 in metazoan genes. We analyzed chicken genes that are developmentally regulated, constitutively active, or inactive. We found that active genes contain high levels of these modifications compared with inactive genes. Furthermore, in actively transcribed regions the levels of di- and tri-Me K36/H3 peak toward the 3′ end of the gene. This is in striking contrast to the distributions of di- and tri-Me K4/H3, which peak early in actively transcribed regions. Thus, di/tri-Me K4/H3 and di/tri-Me K36/H3 are both useful markers of active genes, but their genic distribution indicates differing roles. Our data suggest that the unique spatial distribution of di- and tri-Me K36/H3 plays a role in transcriptional termination and/or early RNA processing.

U2 - 10.1074/jbc.M500796200

DO - 10.1074/jbc.M500796200

M3 - Article

VL - 280

SP - 17732

EP - 17736

JO - The Journal of Biological Chemistry

JF - The Journal of Biological Chemistry

SN - 0021-9258

IS - 18

ER -

ID: 155510