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Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro

Research output: Contribution to journalArticlepeer-review

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Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro. / Twaites, B.; De las Heras Alarcon, C.; Cunliffe, D.; Lavigne, M.; Pennadam, S.; Smith, James; Gorecki, Darek; Alexander, C.

In: Journal of Controlled Release, Vol. 97, No. 3, 2004, p. 551-566.

Research output: Contribution to journalArticlepeer-review

Harvard

Twaites, B, De las Heras Alarcon, C, Cunliffe, D, Lavigne, M, Pennadam, S, Smith, J, Gorecki, D & Alexander, C 2004, 'Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro', Journal of Controlled Release, vol. 97, no. 3, pp. 551-566. https://doi.org/10.1016/j.jconrel.2004.03.032

APA

Twaites, B., De las Heras Alarcon, C., Cunliffe, D., Lavigne, M., Pennadam, S., Smith, J., Gorecki, D., & Alexander, C. (2004). Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro. Journal of Controlled Release, 97(3), 551-566. https://doi.org/10.1016/j.jconrel.2004.03.032

Vancouver

Twaites B, De las Heras Alarcon C, Cunliffe D, Lavigne M, Pennadam S, Smith J et al. Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro. Journal of Controlled Release. 2004;97(3):551-566. https://doi.org/10.1016/j.jconrel.2004.03.032

Author

Twaites, B. ; De las Heras Alarcon, C. ; Cunliffe, D. ; Lavigne, M. ; Pennadam, S. ; Smith, James ; Gorecki, Darek ; Alexander, C. / Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro. In: Journal of Controlled Release. 2004 ; Vol. 97, No. 3. pp. 551-566.

Bibtex

@article{96ccf266b3c3462d9e291350f1cdccf2,
title = "Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro",
abstract = "Poly(N-isopropylacrylamide) (PNIPAm) co-polymers responsive to temperature and pH were prepared with side chain chemistries in order to exhibit phase transitions under physiologically relevant conditions. Fluorescence spectroscopy, gel retardation assays, dynamic light scattering and atomic force microscopy were used to characterize the binding of plasmid DNA to these materials and to control polymers poly(ethyleneimine) (PEI) and poly(ethyleneimine)-octanamide. Complexes of plasmid DNA with thermoresponsive cationic polymers containing PNIPAm displayed variations in gel retardation behaviour above and below polymer phase transition temperatures, with a high molecular weight linear cationic PNIPAm co-polymer forming complexes with reduced affinity above LCST whereas a branched PEI-PNIPAm co-polymer bound with higher affinity above the PNIPAm phase transition. The thermoresponsive polymers also exhibited changes in particle morphology across the same temperature ranges with polymer DNA complexes prepared at N/P ratios of 2:1 generating spherical particles varying in radius between 30–70 nm at 25 °C and 60–100 nm at 40–45 °C. The transport of DNA within these complexes to cell nuclei was demonstrated to occur within 24 h in tissue culture via confocal microscopy, and low level transfection of mouse muscle cells by a reporter gene encoding green fluorescent protein was achieved with the branched thermoresponsive PEI-PNIPAm conjugate.",
author = "B. Twaites and {De las Heras Alarcon}, C. and D. Cunliffe and M. Lavigne and S. Pennadam and James Smith and Darek Gorecki and C. Alexander",
year = "2004",
doi = "10.1016/j.jconrel.2004.03.032",
language = "English",
volume = "97",
pages = "551--566",
journal = "Journal of Controlled Release: Official Journal of the Controlled Release Society",
issn = "0168-3659",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Thermo and pH responsive polymers as gene delivery vectors: effect of polymer architecture on DNA complexation in vitro

AU - Twaites, B.

AU - De las Heras Alarcon, C.

AU - Cunliffe, D.

AU - Lavigne, M.

AU - Pennadam, S.

AU - Smith, James

AU - Gorecki, Darek

AU - Alexander, C.

PY - 2004

Y1 - 2004

N2 - Poly(N-isopropylacrylamide) (PNIPAm) co-polymers responsive to temperature and pH were prepared with side chain chemistries in order to exhibit phase transitions under physiologically relevant conditions. Fluorescence spectroscopy, gel retardation assays, dynamic light scattering and atomic force microscopy were used to characterize the binding of plasmid DNA to these materials and to control polymers poly(ethyleneimine) (PEI) and poly(ethyleneimine)-octanamide. Complexes of plasmid DNA with thermoresponsive cationic polymers containing PNIPAm displayed variations in gel retardation behaviour above and below polymer phase transition temperatures, with a high molecular weight linear cationic PNIPAm co-polymer forming complexes with reduced affinity above LCST whereas a branched PEI-PNIPAm co-polymer bound with higher affinity above the PNIPAm phase transition. The thermoresponsive polymers also exhibited changes in particle morphology across the same temperature ranges with polymer DNA complexes prepared at N/P ratios of 2:1 generating spherical particles varying in radius between 30–70 nm at 25 °C and 60–100 nm at 40–45 °C. The transport of DNA within these complexes to cell nuclei was demonstrated to occur within 24 h in tissue culture via confocal microscopy, and low level transfection of mouse muscle cells by a reporter gene encoding green fluorescent protein was achieved with the branched thermoresponsive PEI-PNIPAm conjugate.

AB - Poly(N-isopropylacrylamide) (PNIPAm) co-polymers responsive to temperature and pH were prepared with side chain chemistries in order to exhibit phase transitions under physiologically relevant conditions. Fluorescence spectroscopy, gel retardation assays, dynamic light scattering and atomic force microscopy were used to characterize the binding of plasmid DNA to these materials and to control polymers poly(ethyleneimine) (PEI) and poly(ethyleneimine)-octanamide. Complexes of plasmid DNA with thermoresponsive cationic polymers containing PNIPAm displayed variations in gel retardation behaviour above and below polymer phase transition temperatures, with a high molecular weight linear cationic PNIPAm co-polymer forming complexes with reduced affinity above LCST whereas a branched PEI-PNIPAm co-polymer bound with higher affinity above the PNIPAm phase transition. The thermoresponsive polymers also exhibited changes in particle morphology across the same temperature ranges with polymer DNA complexes prepared at N/P ratios of 2:1 generating spherical particles varying in radius between 30–70 nm at 25 °C and 60–100 nm at 40–45 °C. The transport of DNA within these complexes to cell nuclei was demonstrated to occur within 24 h in tissue culture via confocal microscopy, and low level transfection of mouse muscle cells by a reporter gene encoding green fluorescent protein was achieved with the branched thermoresponsive PEI-PNIPAm conjugate.

U2 - 10.1016/j.jconrel.2004.03.032

DO - 10.1016/j.jconrel.2004.03.032

M3 - Article

VL - 97

SP - 551

EP - 566

JO - Journal of Controlled Release: Official Journal of the Controlled Release Society

JF - Journal of Controlled Release: Official Journal of the Controlled Release Society

SN - 0168-3659

IS - 3

ER -

ID: 74131