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Thyroid hormone-regulated enhancer blocking: cooperation of CTCF and thyroid hormone receptor

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Thyroid hormone-regulated enhancer blocking: cooperation of CTCF and thyroid hormone receptor. / Lutz, M.; Burke, L.; Le Fevre, P.; Myers, Fiona; Thorne, Alan; Crane-Robinson, Colyn; Bonifer, C.; Filippova, G.; Lobanenkov, V.; Renkawitz, R.

In: EMBO Journal, Vol. 22, No. 7, 01.04.2003, p. 1579-1587.

Research output: Contribution to journalArticle

Harvard

Lutz, M, Burke, L, Le Fevre, P, Myers, F, Thorne, A, Crane-Robinson, C, Bonifer, C, Filippova, G, Lobanenkov, V & Renkawitz, R 2003, 'Thyroid hormone-regulated enhancer blocking: cooperation of CTCF and thyroid hormone receptor', EMBO Journal, vol. 22, no. 7, pp. 1579-1587. https://doi.org/10.1093/emboj/cdg147

APA

Lutz, M., Burke, L., Le Fevre, P., Myers, F., Thorne, A., Crane-Robinson, C., Bonifer, C., Filippova, G., Lobanenkov, V., & Renkawitz, R. (2003). Thyroid hormone-regulated enhancer blocking: cooperation of CTCF and thyroid hormone receptor. EMBO Journal, 22(7), 1579-1587. https://doi.org/10.1093/emboj/cdg147

Vancouver

Author

Lutz, M. ; Burke, L. ; Le Fevre, P. ; Myers, Fiona ; Thorne, Alan ; Crane-Robinson, Colyn ; Bonifer, C. ; Filippova, G. ; Lobanenkov, V. ; Renkawitz, R. / Thyroid hormone-regulated enhancer blocking: cooperation of CTCF and thyroid hormone receptor. In: EMBO Journal. 2003 ; Vol. 22, No. 7. pp. 1579-1587.

Bibtex

@article{f0d0edfdf61a46068a19b70daccedebc,
title = "Thyroid hormone-regulated enhancer blocking: cooperation of CTCF and thyroid hormone receptor",
abstract = "The highly conserved, ubiquitously expressed, zinc finger protein CTCF is involved in enhancer blocking, a mechanism crucial for shielding genes from illegitimate enhancer effects. Interestingly, CTCF-binding sites are often flanked by thyroid hormone response elements (TREs), as at the chicken lysozyme upstream silencer. Here we identify a similar composite site positioned upstream of the human c-myc gene. For both elements, we demonstrate that thyroid hormone abrogates enhancer blocking. Relief of enhancer blocking occurs even though CTCF remains bound to the lysozyme chromatin. Furthermore, chromatin immunoprecipitation analysis of the lysozyme upstream region revealed that histone H4 is acetylated at the CTCF-binding site. Loss of enhancer blocking by the addition of T3 led to increased histone acetylation, not only at the CTCF site, but also at the enhancer and the promoter. Thus, when TREs are adjacent to CTCF-binding sites, thyroid hormone can regulate enhancer blocking, thereby providing a new property for what was previously thought to be constitutive enhancer shielding by CTCF.",
author = "M. Lutz and L. Burke and {Le Fevre}, P. and Fiona Myers and Alan Thorne and Colyn Crane-Robinson and C. Bonifer and G. Filippova and V. Lobanenkov and R. Renkawitz",
year = "2003",
month = apr,
day = "1",
doi = "10.1093/emboj/cdg147",
language = "English",
volume = "22",
pages = "1579--1587",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "7",

}

RIS

TY - JOUR

T1 - Thyroid hormone-regulated enhancer blocking: cooperation of CTCF and thyroid hormone receptor

AU - Lutz, M.

AU - Burke, L.

AU - Le Fevre, P.

AU - Myers, Fiona

AU - Thorne, Alan

AU - Crane-Robinson, Colyn

AU - Bonifer, C.

AU - Filippova, G.

AU - Lobanenkov, V.

AU - Renkawitz, R.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - The highly conserved, ubiquitously expressed, zinc finger protein CTCF is involved in enhancer blocking, a mechanism crucial for shielding genes from illegitimate enhancer effects. Interestingly, CTCF-binding sites are often flanked by thyroid hormone response elements (TREs), as at the chicken lysozyme upstream silencer. Here we identify a similar composite site positioned upstream of the human c-myc gene. For both elements, we demonstrate that thyroid hormone abrogates enhancer blocking. Relief of enhancer blocking occurs even though CTCF remains bound to the lysozyme chromatin. Furthermore, chromatin immunoprecipitation analysis of the lysozyme upstream region revealed that histone H4 is acetylated at the CTCF-binding site. Loss of enhancer blocking by the addition of T3 led to increased histone acetylation, not only at the CTCF site, but also at the enhancer and the promoter. Thus, when TREs are adjacent to CTCF-binding sites, thyroid hormone can regulate enhancer blocking, thereby providing a new property for what was previously thought to be constitutive enhancer shielding by CTCF.

AB - The highly conserved, ubiquitously expressed, zinc finger protein CTCF is involved in enhancer blocking, a mechanism crucial for shielding genes from illegitimate enhancer effects. Interestingly, CTCF-binding sites are often flanked by thyroid hormone response elements (TREs), as at the chicken lysozyme upstream silencer. Here we identify a similar composite site positioned upstream of the human c-myc gene. For both elements, we demonstrate that thyroid hormone abrogates enhancer blocking. Relief of enhancer blocking occurs even though CTCF remains bound to the lysozyme chromatin. Furthermore, chromatin immunoprecipitation analysis of the lysozyme upstream region revealed that histone H4 is acetylated at the CTCF-binding site. Loss of enhancer blocking by the addition of T3 led to increased histone acetylation, not only at the CTCF site, but also at the enhancer and the promoter. Thus, when TREs are adjacent to CTCF-binding sites, thyroid hormone can regulate enhancer blocking, thereby providing a new property for what was previously thought to be constitutive enhancer shielding by CTCF.

U2 - 10.1093/emboj/cdg147

DO - 10.1093/emboj/cdg147

M3 - Article

VL - 22

SP - 1579

EP - 1587

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 7

ER -

ID: 155630