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Transcription regulation of the Type II Restriction-Modification system AhdI

Research output: Contribution to journalArticlepeer-review

  • E. Bogdanova
  • M. Djordjevic
  • I. Papapanagiotou
  • T. Heyduk
  • Geoff Kneale
  • K. Severinov
The Restriction-modification system AhdI contains two convergent transcription units, one with genes encoding methyltransferase subunits M and S and another with genes encoding the controller (C) protein and the restriction endonuclease (R). We show that AhdI transcription is controlled by two independent regulatory loops that are well-optimized to ensure successful establishment in a naive bacterial host. Transcription from the strong MS promoter is attenuated by methylation of an AhdI site overlapping the -10 element of the promoter. Transcription from the weak CR promoter is regulated by the C protein interaction with two DNA-binding sites. The interaction with the promoter-distal high-affinity site activates transcription, while interaction with the weaker promoter-proximal site represses it. Because of high levels of cooperativity, both C protein-binding sites are always occupied in the absence of RNA polymerase, raising a question how activated transcription is achieved. We develop a mathematical model that is in quantitative agreement with the experiment and indicates that RNA polymerase outcompetes C protein from the promoter-proximal-binding site. Such an unusual mechanism leads to a very inefficient activation of the R gene transcription, which presumably helps control the level of the endonuclease in the cell.
Original languageEnglish
Pages (from-to)1429-1442
Number of pages14
JournalNucleic Acids Research
Volume36
Issue number5
DOIs
Publication statusPublished - Mar 2008

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