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Urinary fibrinopeptide-A as a predictive biomarker of exacerbation in asthma

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Urinary fibrinopeptide-A as a predictive biomarker of exacerbation in asthma. / Owen, Jonathan J.; Edgar, Suzanne; Elliott, Scott; Kerley, Sumita; Jones, Thomas Llewelyn; Neville, Daniel; Fogg, Carole; Brown, Thomas; Chauhan, Anoop; Shute, Jan.

In: Respiratory Medicine, Vol. 2, 100021, 01.11.2020.

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@article{4d81e6c1c73541baa2ce0b8f4be10d7e,
title = "Urinary fibrinopeptide-A as a predictive biomarker of exacerbation in asthma",
abstract = "Background: Asthma is a prevalent and potentially life-threatening disease associated with exacerbation and costly hospital admissions. The coagulation cascade is up-regulated in severe asthma and increased fibrinogenesis in the airway may precede exacerbation in moderate asthma.Objective: A longitudinal prospective study to test the hypothesis that levels of urinary fibrinopeptide A (FP-A), a marker of coagulation, increase prior to an exacerbation of asthma. Methods: 24 non-smoking participants with moderate to severe asthma were recruited and followed to exacerbation and to recovery for up to 8 weeks afterwards. Baseline measurements included spirometry, full blood count, atopic status and plasma markers of coagulation. Participants provided daily Peak Expiratory Flow (PEF) readings and three urine samples per week for analysis of FP-A, a specific marker of activation of coagulation. A novel method to concentrate urinary FP-A for immunodetection and quantification was developed. Participants were followed up until exacerbation, when baseline measurements were repeated, and monthly thereafter for 2 months or to recovery. Measurements and main findings: 17 participants exacerbated during the study. Significantly increased concentrations of plasma D-dimer (0.25(0.2-0.42) vs 0.21(0.12-0.29) µg FEU/ml, p=0.02) were found at exacerbation. A peak in urinary FP-A concentration was detected on average 4.2 ± 2 days prior to exacerbation and was significantly (p<0.05) higher than at exacerbation or 7 days later. Urinary FP-A concentrations correlated positively with time to recovery and negatively (p<0.01) with IgE concentration.Conclusion: FP-A is detectable in urine several days before the onset of an asthma exacerbation indicating disordered coagulation preceding asthma exacerbations.",
author = "Owen, {Jonathan J.} and Suzanne Edgar and Scott Elliott and Sumita Kerley and Jones, {Thomas Llewelyn} and Daniel Neville and Carole Fogg and Thomas Brown and Anoop Chauhan and Jan Shute",
note = "12 month embargo. Elsevier.",
year = "2020",
month = jun,
day = "25",
doi = "10.1016/j.yrmex.2020.100021",
language = "English",
volume = "2",
journal = "Respiratory Medicine",
issn = "0954-6111",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Urinary fibrinopeptide-A as a predictive biomarker of exacerbation in asthma

AU - Owen, Jonathan J.

AU - Edgar, Suzanne

AU - Elliott, Scott

AU - Kerley, Sumita

AU - Jones, Thomas Llewelyn

AU - Neville, Daniel

AU - Fogg, Carole

AU - Brown, Thomas

AU - Chauhan, Anoop

AU - Shute, Jan

N1 - 12 month embargo. Elsevier.

PY - 2020/6/25

Y1 - 2020/6/25

N2 - Background: Asthma is a prevalent and potentially life-threatening disease associated with exacerbation and costly hospital admissions. The coagulation cascade is up-regulated in severe asthma and increased fibrinogenesis in the airway may precede exacerbation in moderate asthma.Objective: A longitudinal prospective study to test the hypothesis that levels of urinary fibrinopeptide A (FP-A), a marker of coagulation, increase prior to an exacerbation of asthma. Methods: 24 non-smoking participants with moderate to severe asthma were recruited and followed to exacerbation and to recovery for up to 8 weeks afterwards. Baseline measurements included spirometry, full blood count, atopic status and plasma markers of coagulation. Participants provided daily Peak Expiratory Flow (PEF) readings and three urine samples per week for analysis of FP-A, a specific marker of activation of coagulation. A novel method to concentrate urinary FP-A for immunodetection and quantification was developed. Participants were followed up until exacerbation, when baseline measurements were repeated, and monthly thereafter for 2 months or to recovery. Measurements and main findings: 17 participants exacerbated during the study. Significantly increased concentrations of plasma D-dimer (0.25(0.2-0.42) vs 0.21(0.12-0.29) µg FEU/ml, p=0.02) were found at exacerbation. A peak in urinary FP-A concentration was detected on average 4.2 ± 2 days prior to exacerbation and was significantly (p<0.05) higher than at exacerbation or 7 days later. Urinary FP-A concentrations correlated positively with time to recovery and negatively (p<0.01) with IgE concentration.Conclusion: FP-A is detectable in urine several days before the onset of an asthma exacerbation indicating disordered coagulation preceding asthma exacerbations.

AB - Background: Asthma is a prevalent and potentially life-threatening disease associated with exacerbation and costly hospital admissions. The coagulation cascade is up-regulated in severe asthma and increased fibrinogenesis in the airway may precede exacerbation in moderate asthma.Objective: A longitudinal prospective study to test the hypothesis that levels of urinary fibrinopeptide A (FP-A), a marker of coagulation, increase prior to an exacerbation of asthma. Methods: 24 non-smoking participants with moderate to severe asthma were recruited and followed to exacerbation and to recovery for up to 8 weeks afterwards. Baseline measurements included spirometry, full blood count, atopic status and plasma markers of coagulation. Participants provided daily Peak Expiratory Flow (PEF) readings and three urine samples per week for analysis of FP-A, a specific marker of activation of coagulation. A novel method to concentrate urinary FP-A for immunodetection and quantification was developed. Participants were followed up until exacerbation, when baseline measurements were repeated, and monthly thereafter for 2 months or to recovery. Measurements and main findings: 17 participants exacerbated during the study. Significantly increased concentrations of plasma D-dimer (0.25(0.2-0.42) vs 0.21(0.12-0.29) µg FEU/ml, p=0.02) were found at exacerbation. A peak in urinary FP-A concentration was detected on average 4.2 ± 2 days prior to exacerbation and was significantly (p<0.05) higher than at exacerbation or 7 days later. Urinary FP-A concentrations correlated positively with time to recovery and negatively (p<0.01) with IgE concentration.Conclusion: FP-A is detectable in urine several days before the onset of an asthma exacerbation indicating disordered coagulation preceding asthma exacerbations.

U2 - 10.1016/j.yrmex.2020.100021

DO - 10.1016/j.yrmex.2020.100021

M3 - Article

VL - 2

JO - Respiratory Medicine

JF - Respiratory Medicine

SN - 0954-6111

M1 - 100021

ER -

ID: 21346155