Using Small angle X-ray acattering (SAXS) to characterise the solution conformation and flexibility of matrix metalloproteinases (MMPs)
Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review
Small angle X-ray scattering (SAXS) provides information about the conformation and flexibility of proteins in solution, and hence provides complementary structural information to that obtained from X-ray crystallography and nuclear magnetic resonance spectroscopy. In this chapter, we describe the methods for the preparation of matrix metalloproteinase (MMP) samples for SAXS analyses, and for the acquisition, processing and interpretation of the SAXS data.
|Title of host publication||Matrix metalloproteases|
|Subtitle of host publication||methods and protocols|
|Number of pages||22|
|Publication status||Published - 16 Mar 2017|
|Name||Methods in Molecular Biology|