Skip to content
Back to outputs

Using the polymerase chain reaction to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis

Research output: Contribution to journalArticlepeer-review

Standard

Using the polymerase chain reaction to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis. / Pearce, C.; Zinkevich, Vitaly; Beech, I.; Funjika, V.; Ruiz, A.; Aladawi, A.; Hamish, D.

In: World Journal of Gastroenterology, Vol. 11, No. 45, 2005, p. 7142-7147.

Research output: Contribution to journalArticlepeer-review

Harvard

Pearce, C, Zinkevich, V, Beech, I, Funjika, V, Ruiz, A, Aladawi, A & Hamish, D 2005, 'Using the polymerase chain reaction to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis', World Journal of Gastroenterology, vol. 11, no. 45, pp. 7142-7147.

APA

Pearce, C., Zinkevich, V., Beech, I., Funjika, V., Ruiz, A., Aladawi, A., & Hamish, D. (2005). Using the polymerase chain reaction to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis. World Journal of Gastroenterology, 11(45), 7142-7147.

Vancouver

Pearce C, Zinkevich V, Beech I, Funjika V, Ruiz A, Aladawi A et al. Using the polymerase chain reaction to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis. World Journal of Gastroenterology. 2005;11(45):7142-7147.

Author

Pearce, C. ; Zinkevich, Vitaly ; Beech, I. ; Funjika, V. ; Ruiz, A. ; Aladawi, A. ; Hamish, D. / Using the polymerase chain reaction to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis. In: World Journal of Gastroenterology. 2005 ; Vol. 11, No. 45. pp. 7142-7147.

Bibtex

@article{30134397de534f138cd1ef94d7024c2a,
title = "Using the polymerase chain reaction to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis",
abstract = "AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP. METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE II score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d 1, 3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team. RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications; the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli. CONCLUSION: Our study confirmed that unlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP.",
author = "C. Pearce and Vitaly Zinkevich and I. Beech and V. Funjika and A. Ruiz and A. Aladawi and D. Hamish",
year = "2005",
language = "English",
volume = "11",
pages = "7142--7147",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "45",

}

RIS

TY - JOUR

T1 - Using the polymerase chain reaction to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis

AU - Pearce, C.

AU - Zinkevich, Vitaly

AU - Beech, I.

AU - Funjika, V.

AU - Ruiz, A.

AU - Aladawi, A.

AU - Hamish, D.

PY - 2005

Y1 - 2005

N2 - AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP. METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE II score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d 1, 3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team. RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications; the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli. CONCLUSION: Our study confirmed that unlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP.

AB - AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP. METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE II score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d 1, 3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team. RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications; the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli. CONCLUSION: Our study confirmed that unlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP.

M3 - Article

VL - 11

SP - 7142

EP - 7147

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 45

ER -

ID: 146996