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Validation of the sperm class analyser CASA system for sperm counting in a busy diagnostic semen analysis laboratory

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Validation of the sperm class analyser CASA system for sperm counting in a busy diagnostic semen analysis laboratory. / Dearing, Chey G.; Kilburn, Sally; Lindsay, Kevin S.

In: Human Fertility, Vol. 17, No. 1, 01.03.2014, p. 37-44.

Research output: Contribution to journalArticlepeer-review

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Dearing, Chey G. ; Kilburn, Sally ; Lindsay, Kevin S. / Validation of the sperm class analyser CASA system for sperm counting in a busy diagnostic semen analysis laboratory. In: Human Fertility. 2014 ; Vol. 17, No. 1. pp. 37-44.

Bibtex

@article{04e068f2c769475fa58db8608286be03,
title = "Validation of the sperm class analyser CASA system for sperm counting in a busy diagnostic semen analysis laboratory",
abstract = "Sperm counts have been linked to several fertility outcomes making them an essential parameter of semen analysis. It has become increasingly recognised that Computer-Assisted Semen Analysis (CASA) provides improved precision over manual methods but that systems are seldom validated robustly for use. The objective of this study was to gather the evidence to validate or reject the Sperm Class Analyser (SCA) as a tool for routine sperm counting in a busy laboratory setting. The criteria examined were comparison with the Improved Neubauer and Leja 20-μm chambers, within and between field precision, sperm concentration linearity from a stock diluted in semen and media, accuracy against internal and external quality material, assessment of uneven flow effects and a receiver operating characteristic (ROC) analysis to predict fertility in comparison with the Neubauer method. This work demonstrates that SCA CASA technology is not a standalone {\textquoteleft}black box{\textquoteright}, but rather a tool for well-trained staff that allows rapid, high-number sperm counting providing errors are identified and corrected. The system will produce accurate, linear, precise results, with less analytical variance than manual methods that correlate well against the Improved Neubauer chamber. The system provides superior predictive potential for diagnosing fertility problems.",
keywords = "computer-assisted semen analysis, validation, semen analysis, sperm count, sperm class analyser",
author = "Dearing, {Chey G.} and Sally Kilburn and Lindsay, {Kevin S.}",
year = "2014",
month = mar,
day = "1",
doi = "10.3109/14647273.2013.865843",
language = "English",
volume = "17",
pages = "37--44",
journal = "Human Fertility",
issn = "1464-7273",
publisher = "Informa Healthcare",
number = "1",

}

RIS

TY - JOUR

T1 - Validation of the sperm class analyser CASA system for sperm counting in a busy diagnostic semen analysis laboratory

AU - Dearing, Chey G.

AU - Kilburn, Sally

AU - Lindsay, Kevin S.

PY - 2014/3/1

Y1 - 2014/3/1

N2 - Sperm counts have been linked to several fertility outcomes making them an essential parameter of semen analysis. It has become increasingly recognised that Computer-Assisted Semen Analysis (CASA) provides improved precision over manual methods but that systems are seldom validated robustly for use. The objective of this study was to gather the evidence to validate or reject the Sperm Class Analyser (SCA) as a tool for routine sperm counting in a busy laboratory setting. The criteria examined were comparison with the Improved Neubauer and Leja 20-μm chambers, within and between field precision, sperm concentration linearity from a stock diluted in semen and media, accuracy against internal and external quality material, assessment of uneven flow effects and a receiver operating characteristic (ROC) analysis to predict fertility in comparison with the Neubauer method. This work demonstrates that SCA CASA technology is not a standalone ‘black box’, but rather a tool for well-trained staff that allows rapid, high-number sperm counting providing errors are identified and corrected. The system will produce accurate, linear, precise results, with less analytical variance than manual methods that correlate well against the Improved Neubauer chamber. The system provides superior predictive potential for diagnosing fertility problems.

AB - Sperm counts have been linked to several fertility outcomes making them an essential parameter of semen analysis. It has become increasingly recognised that Computer-Assisted Semen Analysis (CASA) provides improved precision over manual methods but that systems are seldom validated robustly for use. The objective of this study was to gather the evidence to validate or reject the Sperm Class Analyser (SCA) as a tool for routine sperm counting in a busy laboratory setting. The criteria examined were comparison with the Improved Neubauer and Leja 20-μm chambers, within and between field precision, sperm concentration linearity from a stock diluted in semen and media, accuracy against internal and external quality material, assessment of uneven flow effects and a receiver operating characteristic (ROC) analysis to predict fertility in comparison with the Neubauer method. This work demonstrates that SCA CASA technology is not a standalone ‘black box’, but rather a tool for well-trained staff that allows rapid, high-number sperm counting providing errors are identified and corrected. The system will produce accurate, linear, precise results, with less analytical variance than manual methods that correlate well against the Improved Neubauer chamber. The system provides superior predictive potential for diagnosing fertility problems.

KW - computer-assisted semen analysis

KW - validation

KW - semen analysis

KW - sperm count

KW - sperm class analyser

U2 - 10.3109/14647273.2013.865843

DO - 10.3109/14647273.2013.865843

M3 - Article

VL - 17

SP - 37

EP - 44

JO - Human Fertility

JF - Human Fertility

SN - 1464-7273

IS - 1

ER -

ID: 5069104