Visualisation of plasmid DNA molecules containing T15A15 repeat sequences by atomic force microscopy
Research output: Contribution to journal › Article › peer-review
We have used atomic force microscopy to examine the conformations of a DNA plasmid which contains a repeated sequence that is able to form an unusual DNA structure. About 16.8% of plasmid molecules containing the sequence (T15A15)2 adopted a compacted rod-shaped form compared to 89.5% of control molecules without the repeated sequence. The secondary structure- forming sequence therefore changed the conformation of the supercoiled plasmid in the predicted manner. In about 40.2% of plasmids with the (T15A15)2 sequence a short, branched structure was visible. We speculate that this object may represent a non-standard local DNA conformation, and suggest an interpretation of the structure.
| Original language | English |
|---|---|
| Pages (from-to) | 303-311 |
| Number of pages | 9 |
| Journal | Probe Microscopy |
| Volume | 1 |
| Issue number | 4 |
| Publication status | Published - 19 Oct 1999 |
Links
Related information
ID: 12016905
