Skip to content

X-ray structure of a putative reaction intermediate of 5-aminolaevulinic acid dehydratase

Research output: Contribution to journalArticlepeer-review

  • Peter T. Erskine
  • Leighton Coates
  • Danica Butler
  • James H. Youell
  • Amanda A. Brindley
  • Steve P. Wood
  • Martin J. Warren
  • Peter M. Shoolingin-jordan
  • Jonathan B. Cooper
The X-ray structure of yeast 5-aminolaevulinic acid dehydratase, in which the catalytic site of the enzyme is complexed with a putative cyclic intermediate composed of both substrate moieties, has been solved at 0.16 nm (1.6 Å) resolution. The cyclic intermediate is bound covalently to Lys263 with the amino group of the aminomethyl side chain ligated to the active-site zinc ion in a position normally occupied by a catalytic hydroxide ion. The cyclic intermediate is catalytically competent, as shown by its turnover in the presence of added substrate to form porphobilinogen. The findings, combined with those of previous studies, are consistent with a catalytic mechanism in which the C–C bond linking both substrates in the intermediate is formed before the C–N bond.
Original languageEnglish
Pages (from-to)733-738
JournalBiochemical Journal
Volume373
Issue number3
DOIs
Publication statusPublished - 1 Aug 2003

Related information

Relations Get citation (various referencing formats)

ID: 3963164